Pcold 1
SpletpCold-R 20-mer / 50% GC 1 binding site. 336 .. 355 = 20 annealed bases T m = 56°C: lacI 3114 .. 4196 = 1083 bp 360 amino acids = 38.6 kDa. Product: lac repressor. The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). ... SpletpCold™ Vector Set: 1 Set: USD $1241.00: Takara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series …
Pcold 1
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SpletPRO1D+ INSTANT ACTION C-PL. Circular polarizer is a high-quality effect filter made of top class optical glass with Anti-Stain, Water repellent anti-reflection coating. The filter … SpletpCold-SUMO Description A prokaryotic expression vector ( pCold-SUMO) is reformed on the basis of pCold carrier, and the carrier promoter (CS Promoter) is derived from Antarctic eosinophilic bacteria. The protein expression can be …
Splet30. avg. 2024 · pCold 系列大肠杆菌冷休克蛋白表达载体温度诱导型CSPA启动子质粒. 质粒宝. 为了基因更好的表达. 当培养的大肠杆菌的温度充分降低，几乎暂时停止生长大部分的蛋白表达减少，而此时一组蛋白质称为“冷 … SpletThe inducer in pCold is the lower temperature. IPTG is normally used to increase the amount of T7 RNA polymerase in the cell, the T7 RNA polymerase will then bind to the T7 …
SpletpCold ベク ターは大腸菌コールドショック遺伝子の一つである cspA のプロモーターを利用したコールドショック 発現ベクターで、従来の大腸菌発現系と比較して、発現できる確率や発現産物の可溶性度を向上させる ことができます 1）。 低温発現のため、他の大腸菌由来のタンパク質の発現が抑えられ、純度の高い目的 タンパク質を得ることが可能で … SpletpCOLD 1: Analyze: Sequence: Plasmid Type: Other, Cold shock expression vector: Promotor: cspA: Cloning Method: Unknown: Size: 4407: 5' Sequencing 1 Primer: pCold-Fwd: 5' …
SpletpCOLADuet-1 Sequence and Map Bacterial vector with a ColA origin for the co-expression of two genes. Bacterial vector with a ColA origin for the co-expression of two genes. …
http://www.app17.com/c145959/products/d11302703.html raycon charging padSplet02. sep. 2024 · pCold DNA I ～IV 是应用冷休克基因之一的cspA 基因启动子而设计并 可以有效表达蛋白质的冷休克表达载体。 在cspA 启动子下游插入了 lac operator ，可以严紧调 … raycon charging podSplet为什么经过双酶切连接转化后使用Pcold通用引物进行阳性克隆筛选会出现两条带，但用目的基因的上游引物和通用引物的下游p出来只有一条带，这是连上了还是没… 显示全部 . 关注者. 1. raycon ceoSpletpCold ベクターシリーズ：説明書・データシート・ベクター情報、SDS、CoA｜タカラバイオ株式会社. raycon charging instructionsSplet01. sep. 2024 · The pCold-SUMOa plasmid was finally constructed by the (Seamless Cloning Technology) SCT ( Fig. 1 B). pCold-SUMOa contains the same multi cloning sites with pCold TF with the exception of the EcoR I and Pst I, while the SUMO tag contains the latter two restriction sites. raycon clearanceSpletOur pCold TF DNA vector is a fusion cold shock expression vector that expresses trigger factor (TF) chaperone as a soluble fusion tag. Trigger factor is a prokaryotic ribosome … raycon company valueSpletFigure 1. pCold TF DVA :Vector Map GenBank Accession No. AB213654 M 1 3 I G pCold TF DNA （5,769 bp） C olE1 r i A m p l cspA 5ʼUTR lac operator cspA promoter a c I cspA 3ʼUTR Multiple cloning site Factor Xa site Thrombin site HRV 3C Protease site Trigger Factor (TF) His-Tag TEE IV. Storage -20℃ raycon co founder